COLOR CODE FOR ced-3 Nose

Oct. 11, 2012

DHH interpretation

This wild type adult hermaphrodite was prepared for study of cell fates of “undead cells” which failed to undergo programmed cell death in a ced-3 (n717) mutant background. This mutant allele, isolated in the Horvitz lab, was first described by Ellis and Horvitz in a Worm Breeder’s Gazette article in Jan 1982 and in their paper in 1986. The TEM study of the adult mutant was conducted at MRC, and aimed to follow the exact cell fates of many “undead cells” (JG White et al., 1991). This study encompassed four animals, one for the hermaphrodite nose, one for the RVG and anterior ventral cord, another for anterior ventral cord, and one for the posterior ventral cord and tail cells.

Figures 1 and 2 from the White et al paper come from this specimen, ced-3 Nose. Among the interesting features within the print set are four CEM cell dendrites (normally present in adult male nose, but which undergo programmed cell death in the hermaphrodite embryo), and an extra “supernumerary dendrite” associated with each amphid. The left supernumerary dendrite stops short before reaching the amphid channel (see print 85 and Figure 2c in the paper), but on the right side the supernumerary dendrite forms a cilium that enters the amphid channel (see print 56 and Figure 2b).

References

HM Ellis and HR Horvitz (1982) On Death and Dying in C. elegans. WBG Vol. 7, No 1

HM Ellis and HR Horvitz (1986) Genetic control of programmed cell death in the nematode. Cell 44: 817-829.

JG White, E Southgate and N Thomson (1991) On the nature of undead cells in the nematode Caenorhabditis elegans. Phil Trans R Soc Lond B 331: 263-71.

Color codes (many taken directly from White et al 1991 and from his notes)

Red numbers

1 AmSoL

2 AmSoR

3 AmShR

4 AmShL

Green numbers

1 AWBR

2 AWAR

3 AWCR

4 AWCL

5 AWAL

6 AWBL

11 FLPR

14 BAGL

Blue numbers

1 IL1VR

2 IL2VR

3 IL2VL

4 IL1VL

5 IL2L

6 IL1L

7 IL2R

8 IL1R

9 IL2DL

10 IL1DL

11 IL2DR

12 IL1DR

13 ADFR

14 ASER

15 ADLR

16 supernumerary amphid dendrite on right

17 ASIR

18 ASJR

19 ASKR

20 ASHR

21 ADFR

22 ASGR

23 ADLR

24 OLQVR

25 CEPVR

25a CEMVR undead cell

26 OLQVL

27 CEPVL

27a CEMVL undead cell

28 CEPDL

28a CEMDL undead cell

29 OLQDL

30 OLQDR

31 CEPDR

31a CEMDR undead cell

32 ADFL

33 ADLL

34 ASEL

35 ASJL

36 ASKL

37 ASIL

38 ASHL

39 ASGL

40 ADFL

41 ADLL

Pink numbers

12 ILshDR

13 ILshDL

14 ILsoDL

19 ILsoDR

23 ILshR

25 supernumerary amphid dendrite on left

30 ILshL

31 ILsoL

32 ILsoR

36 ILsoVL

39 ILshVL

43 ILshVR

46 ILsoVR

Violet numbers

1 OLshDR

2 OLshDL

3 OLshVR

4 OLshVL

5 URYD

6 OLsoDL

7 OLsoDR

8 URYD

9 CEPshDR

10 ILsoDR

11 CEPshDL

12 ILshDR

13 ILsoDL

14 ILshDL

15 extra dorsal process ending at print 24

16 URA?

17 CEPsoDL

18 CEPsoDR

19 URX? Seems different from wild type

20 URX? Seems different from wild type

21 OLsoR

22 OLshR

23 ILshR

24 URB?

26 OLsoL

27 extra lateral process ending at print 84

28 URB?

29 OLshL

30 ILshL

31 ILsoL

32 ILsoR

33 extra lateral process ending at print 95

35 OLsoVL

36 URA?

37 extra ventral process ending at print 10

38 ILsoVL

39 ILshVL

40 CEPshVL

41 extra ventral process ending at print 59

42 CEPsoVL

43 ILshVR

44 URA?

45 extra ventral process ending at print 17

46 ILsoVR

48 extra ventral process ending at print 92

50 CEPsoVR

51 CEPshVR

52 URA?